Buds Reactivity and Factors Promoting Shoots Proliferation and Rooting of Cashew Seedlings Using <i>in Vitro</i> Tissue Culture Process

Tissue culture techniques are widely used for the mass propagation of many species. In cashew in vitro propagation, some protocols need to be established at this end. The present work was carried out evaluate conditions regeneration seedlings from micropropagation by organogenesis on Benin genotypes. Nodal explants one-month-old greenhouse and cotyledonary nodes germination were purpose. BAP kinetin evaluated alone 2.2 mg/L then combination + 0.2 IBA also evaluated. response axillary bud proliferation obtained with both buds different combinations growth regulators. However, best responses recorded nodes. When used, 80% responded numerous (5 8) (5.75 ± 0.12) good shoot length (6.73 0.3 cm) MS medium containing 150 mL coconut water. Rooting observed NAA (2.5 mg/l) 1/2 40 g/l sucrose.